Composite

Part:BBa_K3301012:Design

Designed by: Maria Lyons, Hailey Ho, Vina Chen, Zoe Hsieh, Josh Dayie   Group: iGEM19_Baltimore_BioCrew   (2019-10-15)


Codon optimized B. theta gene BT2268 with 6xHis tag and T7 promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1789
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1232
    Illegal BsaI.rc site found at 1688
    Illegal SapI site found at 1607


Design Notes

None


Source

Synthesized by IDT

References